RAD51 Antibody / Homologous Recombination Protein Antibody [clone RAD51/2701] (V7491)

RAD51 Antibody Colon Carcinoma IHC. Immunohistochemical staining of FFPE human colon carcinoma demonstrates RAD51 expression using a RAD51 antibody. RAD51, also known as a Homologous Recombination Protein, is a key mediator of DNA double strand break repair and maintenance of genomic stability. Predominantly nuclear staining is observed in neoplastic cells, consistent with the elevated DNA repair activity frequently associated with proliferating tumors. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris buffer containing 1 mM EDTA for 10 to 20 minutes followed by cooling prior to staining. These results support the utility of RAD51 Antibody for investigating homologous recombination, DNA damage responses, and therapeutic resistance in human cancers.

RAD51 Antibody Colon Carcinoma IHC. Immunohistochemical staining of FFPE human colon carcinoma demonstrates RAD51 expression using a RAD51 antibody. RAD51, also known as a Homologous Recombination Protein, is a central mediator of DNA double strand break repair and maintenance of genomic stability. Strong nuclear staining is observed in malignant epithelial cells, consistent with the elevated homologous recombination activity and proliferative capacity characteristic of colorectal tumors. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris buffer containing 1 mM EDTA for 10 to 20 minutes followed by cooling prior to staining. These results support the utility of RAD51 Antibody for investigating DNA damage responses, homologous recombination, and therapeutic resistance in human cancers.

RAD51 Antibody Human Cell Lines WB. Western blot analysis of RAD51 expression using a RAD51 antibody. Lane 1: human HeLa cell lysate. Lane 2: human HepG2 cell lysate. Lane 3: human K562 cell lysate. Lane 4: human A431 cell lysate. Lane 5: human MCF-7 cell lysate. RAD51, also known as a Homologous Recombination Protein, is a key mediator of DNA double strand break repair and genome stability. A prominent band is detected at approximately 37 kDa in all cell lines, consistent with the predicted molecular weight of RAD51. Additional lower molecular weight bands are observed in some lysates and may represent degradation products or alternatively processed forms, while a higher molecular weight species is evident in A431 cells and may reflect post translationally modified or multimeric forms of RAD51. These results support the utility of RAD51 Antibody for studies of homologous recombination, DNA damage responses, and cancer biology.

RAD51 Antibody Protein Microarray Validation. Analysis of a HuProt human protein microarray containing more than 19,000 full length human proteins demonstrates the high specificity of the RAD51 antibody clone RAD51/2701. RAD51, also known as RAD51 Recombinase, is a key DNA repair protein involved in homologous recombination and maintenance of genomic stability. Among all proteins represented on the array, RAD51 exhibited the strongest signal with a Z score of 75.29. The next highest signal corresponded to KLHL1 with a Z score of 48.82, yielding an S score of 26.47 for RAD51. The Z score reflects the signal intensity generated by antibody binding relative to the mean signal of all proteins on the array, while the S score measures the difference between the top ranked target and the next highest signal. These results support the high specificity of the RAD51/2701 mAb and validate its utility for studies of DNA damage responses, homologous recombination, and genome maintenance.

RAD51 Antibody K562 Cells FACS. Flow cytometric analysis of human K562 cells was performed using RAD51 antibody (clone RAD51/2701). RAD51, also known as a Homologous Recombination Protein, is a key mediator of DNA double strand break repair and maintenance of genomic stability. Red histogram: isotype control. Blue histogram: RAD51 antibody. The pronounced rightward shift of the RAD51 antibody signal relative to the isotype control demonstrates specific detection of endogenous RAD51 in K562 cells. These results support the utility of RAD51 Antibody for studies of homologous recombination, DNA damage responses, and therapeutic resistance in cancer cells.

RAD51 Antibody A431 and A549 WB. Western blot analysis of RAD51 expression using RAD51 antibody (clone RAD51/2701). Lane 1: human A431 cell lysate. Lane 2: human A549 cell lysate. RAD51, also known as RAD51 Recombinase, is a central mediator of homologous recombination and DNA double strand break repair that preserves genomic stability. A prominent band is detected at approximately 37 kDa in both cell lines, consistent with the expected molecular weight of RAD51. These results demonstrate endogenous expression of RAD51 in epithelial cancer cells and support the utility of this antibody for studies of DNA repair pathways, genome maintenance, and cellular responses to genotoxic stress.