RAD21 Antibody / Cohesin Complex Protein Antibody (RQ7197)

RAD21 Antibody Human Cancer Cell IF. Immunofluorescent staining of FFPE human SiHa cells using RAD21 Antibody (green) with DAPI nuclear counterstain (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. RAD21 Antibody, a Cohesin Complex Protein Antibody, demonstrates strong nuclear localization in SiHa cells, consistent with the role of RAD21 in the cohesin complex, chromosome organization, sister chromatid cohesion, and regulation of genome stability.

RAD21 Antibody Human Cell WB. Western blot analysis of human HeLa, MOLT4, HEL, and A431 cell lysates using RAD21 Antibody at 0.5 ug/ml. Lane 1: HeLa, Lane 2: MOLT4, Lane 3: HEL, Lane 4: A431. An HRP-conjugated secondary antibody with ECL substrate was used for detection. RAD21 Antibody, a Cohesin Complex Protein Antibody, detects a band at approximately 130 kDa, consistent with the reported migration pattern of RAD21. The observed molecular weight is higher than the predicted 72 kDa size and may reflect post-translational modification and altered SDS-PAGE migration of the cohesin complex protein.

RAD21 Antibody Human Immune Cell FACS. Flow cytometric analysis of human HL60 cells using RAD21 Antibody at 1 ug/million cells. Cells were blocked with goat serum and compared against cells alone (red) and isotype control (green). RAD21 Antibody, a Cohesin Complex Protein Antibody, demonstrates a clear positive shift (blue), confirming RAD21 detection in HL60 cells and supporting its use for studying cohesin complex function, chromosome organization, and genome stability pathways.