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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Rabbit IgG Isotype Control Polyclonal Antibody PE Conjugate (N1001PE)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
N1001PE-100UG | 0.5 mg/ml antibody in PBS with 0.05% sodium azide | 100 ug | 300 | |
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N1001PE-25UG | 0.5 mg/ml antibody in PBS with 0.05% sodium azide | 25 | 130 |
| Species Reactivity | NA |
| Format | PE Conjugate |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Protein A Affinity Chromatography |
| Buffer | 1X PBS, pH 7.4 |
| Applications | Flow Cytometry : assay dependent Immunohistochemistry : assay dependent Immunofluorescence : assay dependent |
| Limitations | This Rabbit IgG isotype control antibody is available for research use only. |
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Rabbit IgG Isotype Control Polyclonal Antibody conjugated to phycoerythrin, or PE, is designed as a negative control reagent for immunoassays employing red-orange fluorescence detection. PE is a phycobiliprotein with exceptionally high molar absorptivity, making it one of the brightest fluorochromes available. By linking the isotype control antibody to PE, researchers obtain a reagent that mimics the optical properties of experimental rabbit antibodies labeled with the same fluorochrome, enabling precise assessment of nonspecific fluorescence.
This Rabbit IgG Isotype Control Polyclonal Antibody PE Conjugate preserves the structural characteristics of rabbit immunoglobulin but does not bind specifically to cellular antigens. Instead, it reports background signals generated by Fc receptor interactions, nonspecific binding to membranes, or PE related autofluorescence. Because PE emits intense fluorescence, even minimal nonspecific binding can appear as positive signal if a matched isotype control is not included. By quantifying that background, the PE conjugated control ensures accurate gating and interpretation in flow cytometry and microscopy.
In flow cytometry, the Rabbit IgG Isotype Control antibody conjugated to PE is critical for establishing compensation parameters in multicolor panels. PEâs emission spectrum overlaps with other fluorochromes, and proper compensation requires a fluorophore-matched isotype control. In immunofluorescence microscopy, the reagent allows investigators to evaluate whether bright red signals correspond to specific antigen labeling or to nonspecific background. The PE conjugated control is also useful in immunohistochemistry, where endogenous pigments can overlap with PE emission and require careful differentiation.
This reagent is particularly valuable in studies of immune cell phenotyping, signaling, and activation, where precise fluorescence quantitation is required. Synonym terms such as rabbit IgG PE isotype control antibody and phycoerythrin conjugated rabbit control antibody broaden product accessibility. By offering consistent detection of background fluorescence, the Rabbit IgG Isotype Control Polyclonal Antibody PE Conjugate helps researchers ensure that red-orange fluorescence signals represent true antigen expression.
NSJ Bioreagents validates the Rabbit IgG Isotype Control antibody conjugated to PE for reproducibility across applications. With this reagent, investigators can reliably separate nonspecific signal from specific labeling, improving the accuracy of fluorescence-based immunoassays.
Generally to be used at the same concentration as the sample being controlled for.
This is purified naive rabbit sera and as such there was no immunogen.
Store the Rabbit IgG isotype control antibody at 4oC. Do not freeze; protect from light.
Isotype control
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