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Home >> Antibodies >> Pyruvate dehydrogenase kinase 2 Antibody / PDK2

Pyruvate dehydrogenase kinase 2 Antibody / PDK2 (F54683)

  Catalog No Formulation Size Price (USD)  
Image F54683-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54683-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE mouse heart tissue with Pyruvate dehydrogenase kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of mouse heart cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9JK42
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Pyruvate dehydrogenase kinase 2 antibody is available for research use only.
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Description

Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Pyruvate dehydrogenase kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 78-107 from the mouse protein was used as the immunogen for the Pyruvate dehydrogenase kinase 2 antibody.

Storage

Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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