Pycard Antibody / Tms1 / mAsc (RQ6434)

Pycard Antibody Mouse Intestine IHC. Immunohistochemical staining of FFPE mouse intestine tissue using PYCARD Antibody / Inflammasome Adaptor Protein Antibody. PYCARD expression is detected predominantly within the cytoplasm of intestinal epithelial cells, consistent with its role as a central adaptor protein in inflammasome assembly, innate immune signaling, and regulation of mucosal inflammatory responses. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to immunostaining.

Pycard Antibody WB. Western blot analysis of 1) rat lung, 2) rat spleen, 3) rat thymus, 4) mouse lung, 5) mouse spleen, and 6) mouse thymus tissue lysates using PYCARD Antibody / Inflammasome Adaptor Protein Antibody. A distinct immunoreactive band is detected at approximately 22 kDa across all tissue lysates, consistent with the predicted molecular weight of PYCARD (ASC), supporting specific detection of the endogenous inflammasome adaptor protein in both rat and mouse tissues.

Pycard Antibody RAW264.7 Cell FACS. Flow cytometric analysis of mouse RAW264.7 cells using PYCARD Antibody / Inflammasome Adaptor Protein Antibody at 1 ug per million cells. A clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) demonstrates specific detection of endogenous PYCARD (ASC) expression. Cells alone are shown in red. Cells were blocked with goat serum prior to antibody incubation to minimize non-specific binding.