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Home >> Antibodies >> Pulmonary Surfactant-Associated Protein D Antibody

Pulmonary Surfactant-Associated Protein D Antibody [clone SFTPD/4362] (V4098)

  Catalog No Formulation Size Price (USD)  
Image V4098-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4098-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4098SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Pulmonary Surfactant-Associated Protein D antibody (clone SFTPD/4362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
SDS-PAGE analysis of purified, BSA-free Surfactant Protein D antibody (clone SFTPD/4362) as confirmation of integrity and purity.
IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4362). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, lambda
Clone Name SFTPD/4362
Purity Protein A/G affinity
UniProt P35247
Localization Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Pulmonary Surfactant-Associated Protein D antibody is available for research use only.
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Description

Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated Collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials.

Application Notes

Optimal dilution of the Pulmonary Surfactant-Associated Protein D antibody should be determined by the researcher.

Immunogen

A recombinant partial protein (within amino acids 241-336) from the human protein was used as the immunogen for the Pulmonary Surfactant-Associated Protein D antibody.

Storage

Aliquot the Pulmonary Surfactant-Associated Protein D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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