Surfactant Protein C Antibody / SP-C Antibody (F54672)

Surfactant Protein C Antibody Western Blot in Human Lung and K562 Cells. Western blot testing of human lung (lane 1) and K562 cell (lane 2) lysates was performed with Surfactant Protein C Antibody. A distinct band is detected at approximately 21 kDa in the K562 sample, consistent with the predicted molecular weight of prosurfactant protein C, while little to no corresponding signal is visible in the lung lane. This result supports detection of pulmonary surfactant-associated protein C by western blot.

Surfactant Protein C Antibody Western Blot in Jurkat Cells. Western blot testing of human Jurkat cell lysate was performed with Surfactant Protein C Antibody. A distinct band is observed at approximately 17 kDa, below the stated predicted molecular weight of ~21 kDa. The lower apparent molecular weight may reflect proteolytic processing of the SFTPC precursor or migration of a processed protein species. This result supports western blot characterization using an SP-C Antibody.

Surfactant Protein C Antibody IHC in Human Lung Adenocarcinoma. Immunohistochemistry of FFPE human lung adenocarcinoma tissue was performed with Surfactant Protein C Antibody following heat-induced epitope retrieval by steaming sections in pH 6 citrate buffer for 20 min and cooling prior to staining. Strong cytoplasmic staining is visible in tumor cells, with heterogeneous staining intensity across the tissue. The staining demonstrates detection of pulmonary surfactant-associated protein C in lung adenocarcinoma tissue.

Surfactant Protein C Antibody Immunofluorescence in HepG2 Cells. Human HepG2 cells were stained with Surfactant Protein C Antibody (green) and counterstained with DAPI (blue). Strong granular cytoplasmic fluorescence surrounds the nuclei, with comparatively little signal in the nuclear compartment. The intracellular staining supports detection of surfactant protein C using an SP-C Antibody by immunofluorescence.