PTH Antibody Immunohistochemistry / Parathyroid Hormone / C-Terminal [clone PTH/1174] (V2810)

Description

Parathyroid hormone (PTH), encoded by the PTH gene, is a peptide hormone produced by endocrine chief cells of the parathyroid glands and plays a critical role in the regulation of systemic calcium and phosphate balance. By acting on bone, kidney, and vitamin D metabolism pathways, PTH maintains mineral homeostasis and supports normal skeletal physiology. PTH Antibody Immunohistochemistry / Parathyroid Hormone enables visualization of PTH protein in formalin-fixed paraffin-embedded tissue sections, supporting immunohistochemistry analysis of parathyroid endocrine cells in histological specimens used for endocrine pathology and research.

PTH Antibody Immunohistochemistry clone PTH/1174 is a mouse monoclonal antibody designed for sensitive immunohistochemistry detection of Parathyroid hormone in tissue sections. In FFPE samples, immunohistochemistry staining typically demonstrates strong cytoplasmic signal in parathyroid chief cells that synthesize and store PTH prior to secretion. This cytoplasmic staining pattern allows clear identification of hormone-producing endocrine cells within parathyroid gland tissue and provides a reliable molecular marker for evaluating endocrine cell populations in histological preparations.

Immunohistochemistry detection of PTH is widely used in surgical pathology to confirm parathyroid origin in tumors of the neck region. Parathyroid adenoma, parathyroid hyperplasia, and parathyroid carcinoma frequently demonstrate strong staining with a PTH antibody, reflecting active hormone production in these lesions. This diagnostic staining pattern helps distinguish parathyroid-derived tumors from thyroid follicular lesions or metastatic neoplasms that may occur in adjacent anatomical locations.

The PTH protein is synthesized as preproparathyroid hormone and undergoes intracellular processing before secretion as the biologically active peptide hormone. Because hormone production occurs within endocrine cells of the parathyroid gland, immunohistochemistry detection using a PTH antibody highlights cytoplasmic localization within these cells. Visualization of this staining pattern assists pathologists and researchers in identifying parathyroid tissue and assessing endocrine tumor specimens in FFPE sections.

This mouse monoclonal antibody clone PTH/1174 recognizes Parathyroid hormone and is suitable for immunohistochemistry detection of PTH expression in tissue sections. A PTH antibody optimized for immunohistochemistry supports studies investigating parathyroid gland biology, endocrine tumor pathology, and the histological distribution of hormone-producing cells in human tissues.

Application Notes

Optimal dilution of the PTH Antibody Immunohistochemistry should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 32-115 of human PTH was used as the immunogen for the Parathyroid Hormone antibody. The epitope of this mAb maps in the C-terminus of PTH.

Storage

Store the PTH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Parathyroid hormone antibody, PTH antibody, Parathyroid hormone immunohistochemistry antibody, PTH immunohistochemistry antibody