PSTPIP1 Antibody / PEST phosphatase-interacting protein 1 Antibody (FY12487)

PSTPIP1 Antibody by FACS in Human JK Cells. Flow cytometry analysis of fixed and permeabilized human JK cells using rabbit polyclonal PSTPIP1 antibody at 1 ug per million cells. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum before primary antibody incubation for 30 minutes at 20oC. DyLight 488-conjugated goat anti-rabbit IgG was used for detection. The histogram compares PSTPIP1 antibody staining (blue), rabbit IgG isotype control (green), and unstained cells (red). The antibody-stained population exhibits a clear rightward fluorescence shift relative to both controls, demonstrating intracellular detection of PEST Phosphatase-Interacting Protein 1.

PSTPIP1 Antibody by WB in Human THP-1 Cell Lysate. Western blot analysis of human THP-1 whole-cell lysate using rabbit polyclonal PSTPIP1 antibody at 0.5 ug/ml. Proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane. Following blocking with 5% non-fat milk, the membrane was incubated with primary antibody overnight at 4oC, followed by HRP-conjugated goat anti-rabbit IgG at 1:5000 and chemiluminescent detection. A prominent band is detected at approximately 50 kDa, consistent with the predicted molecular weight of approximately 48 kDa for PEST Phosphatase-Interacting Protein 1.

PSTPIP1 Antibody by IP in Human THP-1 Cell Lysate. Immunoprecipitation of PEST Phosphatase-Interacting Protein 1 from human THP-1 whole-cell lysate using rabbit polyclonal PSTPIP1 antibody. Lane 1 contains 30 ug of input lysate, lane 2 represents a rabbit IgG control immunoprecipitation, and lane 3 contains protein immunoprecipitated from 500 ug of THP-1 lysate using 2 ug of PSTPIP1 antibody. Following electrophoresis and membrane transfer, Western blot detection was performed using the same antibody at 0.5 ug/ml and an HRP-conjugated light-chain-specific secondary antibody. A prominent band near 50 kDa is detected in the input and antibody immunoprecipitation lanes, with no comparable band in the control IgG lane, demonstrating enrichment of PSTPIP1.