PSME1 Antibody / Immunoproteasome Activator Antibody (RQ8156)

PSME1 Antibody A549 Cell IF. Immunofluorescent staining of FFPE human A549 cells with PSME1 antibody (red). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei are counterstained with DAPI (blue). PSME1 immunoreactivity is observed predominantly within the cytoplasm with weaker nuclear staining, consistent with the expected localization of this immunoproteasome activator that promotes antigen processing, proteasome activity, and Major Histocompatibility Complex class I peptide presentation.

PSME1 Antibody Human, Rat and Mouse WB. Western blot testing of 1) human Raji, 2) human A431, 3) rat liver, 4) rat spleen, 5) rat RH35, 6) mouse liver, 7) mouse spleen, and 8) mouse RAW264.7 lysates with PSME1 antibody. A distinct immunoreactive band is detected at approximately 29 kDa in all samples, consistent with the predicted molecular weight of PSME1. The results demonstrate specific detection of this immunoproteasome activator, which promotes proteasome-mediated antigen processing and generation of peptides for Major Histocompatibility Complex class I presentation.

PSME1 Antibody U937 Cell Flow Cytometry. Flow cytometric analysis of fixed and permeabilized human U937 cells stained with PSME1 antibody at 1 ug per million cells following goat serum blocking. Red: cells alone. Green: isotype control. Blue: PSME1 antibody. The clear rightward shift of the PSME1 antibody histogram relative to the isotype control demonstrates specific intracellular detection of this immunoproteasome activator, which plays a central role in proteasome-mediated antigen processing and Major Histocompatibility Complex class I peptide presentation.

PSME1 Antibody Jurkat Cell IP-WB. Immunoprecipitation of PSME1 from human Jurkat whole cell lysate followed by western blot analysis using PSME1 antibody. Lane 1: Input Jurkat whole cell lysate (30 ug). Lane 2: Immunoprecipitation using rabbit control IgG (negative control). Lane 3: Immunoprecipitation with 2 ug PSME1 antibody from 500 ug Jurkat whole cell lysate. A specific immunoreactive band is detected at approximately 29 kDa, consistent with the predicted molecular weight of PSME1. The enrichment of PSME1 in the immunoprecipitated sample, with no corresponding band in the IgG control, demonstrates the suitability of this immunoproteasome activator antibody for immunoprecipitation and western blot applications.

PSME1 Antibody U2OS Cell IF. Immunofluorescent staining of U2OS cells with PSME1 antibody (green) at 5 ug/ml. Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Nuclei are counterstained with DAPI (blue). PSME1 immunoreactivity is observed predominantly within the cytoplasm with weaker nuclear staining, consistent with the expected localization of this immunoproteasome activator that regulates proteasome activity, antigen processing, and Major Histocompatibility Complex class I peptide generation.

PSME1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue with PSME1 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. PSME1 immunoreactivity is observed predominantly within the cytoplasm and nuclei of malignant epithelial cells, consistent with the expected localization of this immunoproteasome activator that enhances proteasome-mediated antigen processing and Major Histocompatibility Complex class I peptide presentation. Cell nuclei are counterstained with hematoxylin (blue).