pS2 Antibody for IF / TFF1 Immunofluorescence Antibody [clone TFF1/2133] (V7354)

pS2 Antibody for IF. Immunofluorescence analysis of Trefoil factor 1 (TFF1) in PFA-fixed human MCF7 breast cancer cells using pS2 Antibody for IF clone TFF1/2133. Cells show cytoplasmic green fluorescence consistent with intracellular localization of the secreted TFF1 protein, while nuclei are counterstained with Reddot (red). The fluorescence pattern highlights epithelial tumor cells with cytoplasmic distribution typical of secretory pathway proteins and reflects the known estrogen-regulated expression of pS2 in MCF7 breast carcinoma cells.

pS2 Antibody for IF. Immunofluorescence analysis of Trefoil factor 1 (TFF1) in PFA-fixed human MCF7 breast cancer cells using pS2 Antibody for IF clone TFF1/2133. Green fluorescence indicates cytoplasmic localization of TFF1 within epithelial tumor cells, consistent with intracellular trafficking of this secreted trefoil family peptide. The actin cytoskeleton is visualized with Phalloidin (red), outlining cell boundaries and cytoplasmic architecture, allowing clear visualization of TFF1 immunofluorescence signal within individual MCF7 cells.

Flow cytometry staining of PFA-fixed human MCF7 cells with pS2 antibody; Red=isotype control, Blue= TFF1/2133 antibody.

IHC staining of FFPE human breast carcinoma with pS2 antibody (clone TFF1/2133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using pS2 antibody (clone TFF1/2133). These results demonstrate the foremost specificity of the TFF1/2133 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.