PRRG1 Antibody / TMG1 Polyclonal Antibody (RQ8024)

PRRG1 Antibody by IHC in human cerebral cortex. Immunohistochemistry of FFPE human cerebral cortex tissue using PRRG1 Antibody at 2 ug/ml. The image shows widespread light-to-moderate brown staining throughout the neuropil, with stronger staining in scattered cellular processes and some neuronal cell bodies. Numerous nuclei are counterstained blue, while vascular profiles and surrounding tissue architecture remain distinguishable. The staining is predominantly extranuclear and appears broadly distributed across the cortical tissue. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. The observed pattern demonstrates PRRG1 immunoreactivity in neural tissue using this TMG1 Polyclonal Antibody.

PRRG1 Antibody by WB in HepG2, A431 and PC-3 cells. Western blot analysis of human cell lysates using PRRG1 Antibody. Lane 1: HepG2; lane 2: A431; lane 3: PC-3. A distinct immunoreactive band is visible in all three lanes at approximately 28-30 kDa, with the strongest signal in A431 cells. The predicted molecular weight of PRRG1 is approximately 25 kDa. Migration slightly above the calculated size is consistent with the behavior observed in the additional six-sample WB validation and may reflect post-translational modification or differences in electrophoretic mobility. The relatively clean banding pattern supports detection of the expected PRRG1 protein using this TMG1 Polyclonal Antibody.

PRRG1 Antibody by WB in human cell lines, rat C6 cells and mouse spinal cord. Western blot analysis of PRRG1 using rabbit polyclonal antibody. Samples were separated by 12% SDS-PAGE under reducing conditions, with 30 ug of protein loaded per lane. Lane 1: human HepG2 cells; lane 2: human A431 cells; lane 3: human Caco-2 cells; lane 4: human PC-3 cells; lane 5: rat C6 cells; lane 6: mouse spinal cord. A prominent band is detected at approximately 28-30 kDa in the human samples, with weaker signals in rat and mouse samples. The predicted molecular weight is approximately 25 kDa. The modest upward migration may reflect post-translational modifications or electrophoretic behavior of this membrane-associated protein. This TMG1 Polyclonal Antibody was used at 0.5 ug/ml.

PRRG1 Antibody by IF in human SiHa cells. Immunofluorescence staining of human SiHa cells using PRRG1 Antibody at 5 ug/ml. Green fluorescence indicates PRRG1 immunoreactivity, while DAPI counterstaining identifies nuclei in blue. The image shows relatively weak, diffuse green staining in the extranuclear cellular regions, with occasional brighter peripheral and punctate signals. Nuclear regions exhibit little green fluorescence. The distribution is compatible with an extranuclear protein, although a distinct plasma membrane localization cannot be established from this image alone. Cells underwent enzymatic antigen retrieval and overnight primary antibody incubation at 4oC, followed by a Fluoro488-conjugated secondary antibody. This TMG1 Polyclonal Antibody provides an additional means of examining PRRG1 localization.