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Home >> Antibodies >> PRPF4 Antibody / U4/U6 small nuclear ribonucleoprotein Prp4

PRPF4 Antibody / U4/U6 small nuclear ribonucleoprotein Prp4 (RQ8179)

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  Catalog No Formulation Size Price (USD)  
Image RQ8179 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with PRPF4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with PRPF4 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HepG2 and 3) 293T cell lysate with PRPF4 antibody. Predicted molecular weight ~58 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with PRPF4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O43172
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PRPF4 antibody is available for research use only.
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Description

U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. he protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the PRPF4 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R5-H461) was used as the immunogen for the PRPF4 antibody.

Storage

After reconstitution, the PRPF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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