• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Proteasome subunit beta type-5 Antibody / PSMB5 / MB1

Proteasome subunit beta type-5 Antibody / PSMB5 / MB1 (RQ6433)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ6433 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
›
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Proteasome subunit beta type-5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A549, 4) rat liver, 5) rat pancreas, 6) rat testis, 7) rat kidney, 8) mouse liver, 9) mouse testis and 10) mouse kidney tissue lysate with Proteasome subunit beta type-5 antibody. Predicted molecular weight: 18-28 kDa (multiple isoforms).
Flow cytometry testing of human U-87 MG cells with Proteasome subunit beta type-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Proteasome subunit beta type-5 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P28074
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Proteasome subunit beta type-5 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Proteasome subunit beta type-5 as known as 20S proteasome subunit beta-5 is a protein that in humans is encoded by the PSMB5 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Proteasome subunit beta type-5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-P263) was used as the immunogen for the Proteasome subunit beta type-5 antibody.

Storage

After reconstitution, the Proteasome subunit beta type-5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all PSMB5 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.