Prostate Specific Antigen Antibody for WB / PSA KLK3 Western Blot Antibody [clone RM323] (R20347)

Prostate Specific Antigen Antibody for WB western blot analysis of PSA and PSA-ACT complex. Western blot testing of purified PSA (from human seminal fluid), ACT (alpha 1-antichymotrypsin from human plasma), and the PSA-ACT complex under non-reducing (-) and reducing (+) conditions using Prostate Specific Antigen Antibody for WB (clone RM323) at 1:2500 shows a strong band corresponding to PSA at approximately 30-34 kDa. Under non-reducing conditions, a higher molecular weight band consistent with the PSA-ACT complex is observed near approximately 90 kDa, while the ACT control alone does not produce a detectable PSA band. Reduction disrupts the complex and results in detection of the PSA protein at its expected molecular weight. Ponceau S staining of the membrane is shown at left to confirm protein loading prior to immunoblot detection.

IHC staining of FFPE human prostate cancer tissue with recombinant Prostate Specific Antigen antibody at 1:1000.

A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of recombinant Prostate Specific Antigen antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.