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Home >> Antibodies >> Prostate Specific Antigen Antibody / PSA

Prostate Specific Antigen Antibody / PSA [clone rKLK3/6947] (V4646)

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  Catalog No Formulation Size Price (USD)  
Image V4646-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4646-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4646SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human prostate carcinoma tissue with Prostate Specific Antigen antibody (clone rKLK3/6947). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate carcinoma tissue with Prostate Specific Antigen antibody (clone rKLK3/6947). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Prostate Specific Antigen antibody (clone rKLK3/6947) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rKLK3/6947
Purity Protein A/G affinity
UniProt P07288
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Prostate Specific Antigen antibody is available for research use only.
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Description

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This mAb makes an excellent pair with mAb 1A7G6B6 for PSA tests.

Application Notes

Optimal dilution of the Prostate Specific Antigen antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 150-250) from the human protein was used as the immunogen for the Prostate Specific Antigen antibody.

Storage

Aliquot the Prostate Specific Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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