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Home >> Antibodies >> Prostasin Antibody / PRSS8

Prostasin Antibody / PRSS8 (RQ8101)

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  Catalog No Formulation Size Price (USD)  
Image RQ8101 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human MCF7 cells with Prostasin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prostasin antibody.
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Flow cytometry testing of fixed and permeabilized human MCF7 cells with Prostasin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prostasin antibody.
Western blot testing of human 1) Caco-2, 2) MCF7 and 3) placental tissue lysate with Prostasin antibody. Predicted molecular weight ~36 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q16651
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Prostasin antibody is available for research use only.
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Description

Prostasin is a protein that in humans is encoded by the PRSS8 gene This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation.

Application Notes

Optimal dilution of the Prostasin antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q59-R322) was used as the immunogen for the Prostasin antibody.

Storage

After reconstitution, the Prostasin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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