Prostaglandin reductase 1 Antibody / PTGR1 (RQ8381)

Prostaglandin Reductase 1 Antibody RT4 Cell FACS. Flow cytometry analysis of fixed and permeabilized human RT4 cells using Prostaglandin Reductase 1 Antibody demonstrates a clear rightward shift of the positive cell population (blue) relative to the isotype control (green), consistent with intracellular expression of PTGR1. Prostaglandin Reductase 1 is a NADP-dependent oxidoreductase involved in prostaglandin and leukotriene metabolism, helping regulate eicosanoid signaling pathways associated with inflammation and lipid mediator turnover. Cells were blocked with goat serum prior to staining. Red=cells alone; Green=isotype control; Blue=Prostaglandin Reductase 1 antibody.

Prostaglandin Reductase 1 Antibody Western Blot. Western blot analysis of human HeLa (lane 1), HepG2 (lane 2), A549 (lane 3), RT4 (lane 4), SiHa (lane 5), HaCaT (lane 6), Caco-2 (lane 7), and PC-3 (lane 8) cell lysates using Prostaglandin Reductase 1 Antibody demonstrates bands at approximately 33-36 kDa, consistent with the predicted molecular weights of the two known PTGR1 isoforms. PTGR1 is a NADP-dependent oxidoreductase involved in prostaglandin and leukotriene metabolism, contributing to the regulation of eicosanoid signaling pathways associated with inflammation, oxidative stress responses, and lipid mediator turnover. Detection of the expected molecular weight bands across multiple human cell lines supports expression of PTGR1 and the suitability of this antibody for western blot applications.