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Home >> Antibodies >> Prostaglandin E synthase 3 Antibody / PTGES3

Prostaglandin E synthase 3 Antibody / PTGES3 [clone 9D3D1] (RQ7027)

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  Catalog No Formulation Size Price (USD)  
Image RQ7027 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human T-47D cells with Prostaglandin E synthase 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human T-47D cells with Prostaglandin E synthase 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human COLO-320, 4) human K562, 5) rat liver, 6) rat RH35 and 7) mouse HEPA1-6 cell lysate with Prostaglandin E synthase 3 antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human PC-3 cells with Prostaglandin E synthase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prostaglandin E synthase 3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 9D3D1
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q15185
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1 ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Prostaglandin E synthase 3 antibody is available for research use only.
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  • Applications : WB, FACS, IF, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

Prostaglandin E synthase 3 (cytosolic) is an enzyme that in humans is encoded by the PTGES3 gene. It is mapped to 12q13.3; 12. This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.

Application Notes

Optimal dilution of the Prostaglandin E synthase 3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-K79) was used as the immunogen for the Prostaglandin E synthase 3 antibody.

Storage

After reconstitution, the Prostaglandin E synthase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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