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Home >> Antibodies >> Proliferation Marker Antibody

Proliferation Marker Antibody [clone JC1] (V8296)

  Catalog No Formulation Size Price (USD)  
Image V8296-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8296-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8296SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human lymph node with Proliferation Marker antibody (clone JC1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node with Proliferation Marker antibody (clone JC1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name JC1
Purity Protein G affinity chromatography
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Proliferation Marker antibody is available for research use only.
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Description

JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

Application Notes

Optimal dilution of the Proliferation Marker antibody should be determined by the researcher.

Immunogen

Pokeweed-stimulated lymphocytes were used as the immunogen for this Proliferation Marker antibody.

Storage

Store the Proliferation Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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