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Home >> Antibodies >> Proliferating Cell Nuclear Antigen Antibody / PCNA

Proliferating Cell Nuclear Antigen Antibody / PCNA [clone PCNA/8303R] (V4803)

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  Catalog No Formulation Size Price (USD)  
Image V4803-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4803-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4803SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with Proliferating Cell Nuclear Antigen antibody (clone PCNA/8303R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with Proliferating Cell Nuclear Antigen antibody (clone PCNA/8303R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with Proliferating Cell Nuclear Antigen antibody (clone PCNA/8303R). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
SDS-PAGE analysis of purified, BSA-free Proliferating Cell Nuclear Antigen antibody (clone PCNA/8303R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PCNA/8303R
Purity Protein A/G affinity
UniProt P12004
Localization Nucleus, Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Proliferating Cell Nuclear Antigen antibody is available for research use only.
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Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

Application Notes

Optimal dilution of the Proliferating Cell Nuclear Antigen antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Proliferating Cell Nuclear Antigen antibody.

Storage

Aliquot the Proliferating Cell Nuclear Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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