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- Tel: 858.663.9055
- Email: info@nsjbio.com
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It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.
The optimal dilution of the Prolactin Receptor antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Semi-purified human prolactin receptor (B6.2) and recombinant human protein (PRLR742) were used as the immunogens for this Prolactin Receptor antibody cocktail.
Store the Prolactin Receptor antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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