Prohibitin Antibody / PHB [clone PHB/3194] (V7596)

Description

Prohibitin/PHB antibody targets Prohibitin, a highly conserved protein that plays important roles in mitochondrial integrity, cellular signaling, and regulation of cell proliferation. Prohibitin is encoded by the PHB gene and is best known as a scaffold protein within the inner mitochondrial membrane, where it forms large ring-like complexes that contribute to maintenance of mitochondrial structure and stability. Through these complexes, Prohibitin supports proper organization of mitochondrial cristae and helps preserve mitochondrial function under both basal and stress conditions.

Prohibitin is also referred to in the literature as PHB and has been studied extensively for its multifunctional roles beyond mitochondria. While a substantial pool of Prohibitin localizes to mitochondria, additional populations have been reported in the cytoplasm and nucleus, where Prohibitin participates in regulation of transcription, signal transduction, and cell cycle control. These diverse localizations reflect the protein’s ability to interact with multiple signaling partners and regulatory complexes. Use of a Prohibitin antibody therefore supports investigation of both mitochondrial and extra-mitochondrial functions of Prohibitin in cell-based systems.

In mitochondrial biology, Prohibitin functions as a stabilizing factor for respiratory chain components and mitochondrial membrane proteins. Loss or dysregulation of PHB has been associated with altered mitochondrial morphology, impaired oxidative phosphorylation, and increased susceptibility to cellular stress. Because mitochondrial dysfunction is a hallmark of many diseases, Prohibitin has attracted attention in studies of neurodegeneration, metabolic disorders, and aging. Prohibitin antibody is frequently applied to examine changes in mitochondrial organization and stress responses linked to PHB expression.

Prohibitin also plays context-dependent roles in cancer biology. Depending on cell type and signaling environment, PHB has been reported to exhibit tumor suppressive or pro-survival functions through interactions with oncogenic signaling pathways and transcriptional regulators. These findings have made Prohibitin an important research target for understanding how mitochondrial signaling interfaces with nuclear control of cell growth and survival. Detection of Prohibitin expression provides insight into these regulatory networks across normal and disease-associated models.

Prohibitin antibody (clone PHB/3194) is designed to detect Prohibitin in research applications. Analysis of PHB expression and subcellular distribution enables investigation of mitochondrial structure, signaling pathways, and cellular stress responses. Overall, Prohibitin remains a widely studied protein linking mitochondrial function with broader cellular regulatory processes.

Application Notes

Optimal dilution of the Prohibitin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.

Storage

Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).