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Home >> Antibodies >> Prohibitin Antibody / PHB

Prohibitin Antibody / PHB (F48122)

  Catalog No Formulation Size Price (USD)  
Image F48122-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48122-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Prohibitin antibody western blot analysis in HepG2 lysate. Predicted molecular weight ~29 kDa.
IHC analysis of FFPE human hepatocarcinoma with Prohibitin antibody
Flow cytometric analysis of ATDC5 cells using Prohibitin antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P35232
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This Prohibitin antibody is available for research use only.
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Description

Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.

Application Notes

Titration of the Prohibitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 92-121 from the human protein was used as the immunogen for this Prohibitin antibody.

Storage

Aliquot the Prohibitin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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