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Home >> Antibodies >> Progesterone Antibody

Progesterone Antibody [clone 6-5E-3F] (V3055)

  Catalog No Formulation Size Price (USD)  
Image V3055-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V3055-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V3055SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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SDS-PAGE Analysis of Purified, BSA-Free Progesterone Antibody (clone 6-5E-3F). Confirmation of Integrity and Purity of the Antibody.
Molecular Structure of Progesterone
Availability 1-3 business days
Species Reactivity All species
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 6-5E-3F
Purity Protein G affinity chromatography
Localization Secreted and cytoplasmic
Applications ELISA (order BSA/sodium Azide-free Format For Coating) :
Radioimmnoassay (RIA) :
Limitations This Progesterone antibody is available for research use only.
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Description

Progesterone Antibody, clone 6-5E-3F, is a monoclonal antibody developed for detection of progesterone, an important steroid hormone involved in endocrine signaling, reproductive biology and pregnancy-associated processes. Specificity testing by ELISA demonstrates selective recognition of progesterone with limited cross-reactivity against related steroid compounds. Relative antibody reactivity was observed with Progesterone-11a-HMS-BSA (100%), 5-beta-Pregnane-3,20-dione (48%), 5-alpha-Pregnane-3,20-dione (26.4%), 17-alpha-Hydroxyprogesterone (2.5%) and 20-alpha-Hydroxyprogesterone (0.04%).

Progesterone belongs to the steroid hormone family and is produced through biosynthetic pathways beginning with cholesterol conversion to pregnenolone. It is generated by several endocrine tissues, including the corpus luteum, adrenal cortex and placenta. Progesterone functions as a major regulator of female reproductive physiology by supporting uterine preparation, endometrial changes and biological pathways required for pregnancy maintenance.

Studies of progesterone provide important insight into hormone regulation, ovarian function and communication between endocrine tissues. In normal ovarian physiology, luteinized theca and granulosa cells contribute to progesterone production following ovulation. Analysis of progesterone levels and activity is widely used in reproductive biology research, endocrine pathway studies and investigations of hormone-dependent cellular responses.

Progesterone Antibody, clone 6-5E-3F, provides researchers with a tool for studying progesterone biology and steroid hormone regulation. NSJ Bioreagents offers this Progesterone Antibody for research involving reproductive endocrinology, ovarian biology, hormone signaling pathways and steroid hormone analysis.

Application Notes

Optimal dilution of the Progesterone antibody should be determined by the researcher.

Immunogen

Progesterone hemisuccinate conjugated to bovine serum albumin was used as the immunogen for the Progesterone antibody.

Storage

Store the Progesterone antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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