PRND Antibody / Prion-like protein doppel (FY13436)

Immunohistochemical staining using PRND antibody on paraffin-embedded human testis tissue sections. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8.0) prior to antibody incubation. Sections were blocked with 10% goat serum and incubated with PRND antibody overnight at 4C. Detection was carried out using an HRP-based secondary antibody with DAB as the chromogen. Staining demonstrates membranous and cytoplasmic immunoreactivity within seminiferous tubules. Nuclei were counterstained with hematoxylin.

Immunofluorescence staining using PRND antibody on paraffin-embedded human testis tissue sections. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8.0) prior to antibody incubation. Sections were blocked with 10% goat serum and incubated with PRND antibody overnight at 4C. Fluorescent signal was detected using a Cy3-conjugated secondary antibody, and nuclei were counterstained with DAPI. Staining highlights PRND expression within cells of the seminiferous tubules.

Immunofluorescence staining using PRND antibody in cultured U2OS cells. Enzyme-mediated antigen retrieval was performed prior to antibody incubation. Cells were blocked with 10% goat serum and incubated with PRND antibody overnight at 4C. Fluorescent signal was detected using a DyLight 488-conjugated secondary antibody, and nuclei were counterstained with DAPI. Staining shows cytoplasmic immunoreactivity in U2OS cells.

Western blot analysis of PRND using PRND antibody. Lane 1: rat testis tissue lysate; Lane 2: rat spleen tissue lysate; Lane 3: rat C6 whole cell lysate; Lane 4: mouse testis tissue lysate. A prominent band is detected at approximately 20 kDa, consistent with the expected molecular weight of Prion-like protein doppel. In addition, a lower molecular weight band around ~15 kDa and higher molecular weight species between ~60-80 kDa can be observed in a tissue-dependent manner, which may reflect processed, glycosylated, or mature forms of the GPI-anchored PRND protein.

Flow cytometry analysis of fixed human U-2 OS cells with PRND antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRND antibody.