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Home >> Antibodies >> PRMT5 Antibody

PRMT5 Antibody (RQ5621)

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  Catalog No Formulation Size Price (USD)  
Image RQ5621 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human 293T cells with PRMT5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRMT5 antibody.
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Flow cytometry testing of human 293T cells with PRMT5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRMT5 antibody.
Western blot testing of 1) human HL-60, 2) rat thymus and 3) mouse kidney lysate with PRMT5 antibody. Expected molecular weight ~72 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O14744
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PRMT5 antibody is available for research use only.
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Description

Protein arginine N-methyltransferase 5 is an enzyme that in humans is encoded by the PRMT5 gene. It is mapped to 14q11.2. This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the PRMT5 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids R18-A552) was used as the immunogen for the PRMT5 antibody.

Storage

After reconstitution, the PRMT5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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