PRKD2 Antibody / Serine Threonine Kinase Antibody (FY13466)

PRKD2 Antibody SiHa Cell IF. Immunofluorescent staining of SiHa cells with PRKD2 antibody (green) at 5 ug/ml. Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Nuclei are counterstained with DAPI (blue). PRKD2 immunoreactivity is observed predominantly within the cytoplasm, consistent with the expected localization of this serine threonine kinase that regulates intracellular signaling, vesicle trafficking, cell survival, and proliferation.

PRKD2 Antibody Human and Mouse WB. Western blot testing of 1) human HEL, 2) human RT4, 3) human SiHa, and 4) mouse thymus lysates with PRKD2 antibody at 0.5 ug/ml. A prominent immunoreactive band is detected at approximately 105 kDa in all samples, consistent with the expected migration of PRKD2 despite its predicted molecular weight of approximately 97 kDa. The results demonstrate specific detection of this serine threonine kinase, which functions in intracellular signaling pathways regulating cell proliferation, survival, vesicle trafficking, and stress responses.

PRKD2 Antibody Human HEL IP-WB. Immunoprecipitation of PRKD2 from human HEL whole cell lysate followed by western blot analysis using PRKD2 antibody. Lane 1: Input HEL whole cell lysate (30 ug). Lane 2: Immunoprecipitation using rabbit control IgG (negative control). Lane 3: Immunoprecipitation with 2 ug PRKD2 antibody from 500 ug HEL whole cell lysate. A specific immunoreactive band is detected at approximately 105 kDa, consistent with the expected migration of PRKD2 despite its predicted molecular weight of approximately 97 kDa. The enrichment of PRKD2 in the immunoprecipitated sample, with no corresponding band in the IgG control, demonstrates the suitability of this serine threonine kinase antibody for immunoprecipitation and western blot applications.