Protein Kinase C alpha Antibody / PRKCA Rabbit Polyclonal Antibody (F54688)

Protein Kinase C alpha Antibody by IHC in mouse brain tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse brain using PRKCA Rabbit Polyclonal Antibody demonstrates brown immunoreactivity in neuronal cell bodies and surrounding tissue. Staining is particularly evident in the cytoplasm of several large cells, with additional immunoreactivity distributed throughout the section. Hematoxylin counterstaining highlights cell nuclei. Heat-induced epitope retrieval was performed by steaming the section in citrate buffer, pH 6.0, for 20 minutes, followed by cooling before antibody incubation. The staining illustrates PKC alpha expression in mouse brain tissue.

Protein Kinase C alpha Antibody by WB in mouse Neuro-2a cells. Western blot analysis of mouse Neuro-2a neuroblastoma cell lysate using PRKCA Rabbit Polyclonal Antibody demonstrates a distinct immunoreactive band migrating near 77 kDa, consistent with the predicted molecular weight of protein kinase C alpha. The blot contains a single sample lane, with the principal band positioned slightly above the 72 kDa molecular weight marker and below the 95 kDa marker. This migration pattern is consistent with detection of full-length PRKCA. The result demonstrates recognition of the endogenous kinase in a mouse neuronal cell model commonly used to investigate cellular signaling, differentiation, and neurobiological processes.

Protein Kinase C alpha Antibody by WB in human Jurkat cells. Western blot analysis of human Jurkat T lymphocyte cell lysate using PRKCA Rabbit Polyclonal Antibody demonstrates a prominent immunoreactive band near the predicted molecular weight of approximately 77 kDa. The single sample lane shows a distinct band migrating between the 72 and 95 kDa molecular weight markers, consistent with detection of full-length protein kinase C alpha. The observed signal supports recognition of PRKCA in a human lymphoid cell line. This result complements the mouse Neuro-2a western blot and illustrates antibody reactivity with the kinase in cellular models representing different species and tissue origins.

Protein Kinase C alpha Antibody by FACS in mouse Neuro-2a cells. Flow cytometry analysis of mouse Neuro-2a cells using PRKCA Rabbit Polyclonal Antibody demonstrates two distinct fluorescence distributions. The antibody-stained population appears at higher fluorescence intensity than the isotype control, indicating detectable PKC alpha immunoreactivity in the tested cells. The histogram shows separation between the lower-intensity control population and the higher-intensity antibody-stained population. This result supports recognition of PRKCA in mouse Neuro-2a cells under the reported flow cytometry conditions.