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Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. [UniProt]
The stated application concentrations are suggested starting points. Titration of the PRKCA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 1-30 from the mouse protein was used as the immunogen for the PRKCA antibody.
Aliquot the PRKCA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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