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Home >> Antibodies >> PREX1 Antibody

PREX1 Antibody (F54365)

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  Catalog No Formulation Size Price (USD)  
Image F54365-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54365-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with PREX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) MCF7, 2) SK-BR-3 and 3) ThP1 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
Western blot testing of human NCI-H460 cell lysate with PREX1 antibody. Predicted molecular weight ~186 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with PREX1 antibody; Blue=isotype control, Green= PREX1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q8TCU6
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This PREX1 antibody is available for research use only.
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Description

The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PREX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1134-1163 from the human protein was used as the immunogen for the PREX1 antibody.

Storage

Aliquot the PREX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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