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Home >> Antibodies >> PRDX3 Antibody / Peroxiredoxin 3

PRDX3 Antibody / Peroxiredoxin 3 (F54798)

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  Catalog No Formulation Size Price (USD)  
Image F54798-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
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F54798-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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IHC testing of FFPE human lung carcinoma tissue with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human lung carcinoma tissue with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) NCI-H460, 2) MDA-MB-231 and 3) HepG2 cell lysate with PRDX3 antibody. Predicted molecular weight ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PRDX3 antibody.
Flow cytometry testing of human MDA-MB-468 cells with PRDX3 antibody; Blue=isotype control, Green= PRDX3 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P30048
Localization Cytoplasmic
Applications Western Blot : 1:500-1:1000
Flow Cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This PRDX3 antibody is available for research use only.
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Description

PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. This protein is involved in redox regulation of the cell and Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. It acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PRDX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 66-94 from the human protein was used as the immunogen for the PRDX3 antibody.

Storage

Aliquot the PRDX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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