PRAK Antibody / p38-Regulated Stress Kinase (R36080)

PRAK Antibody Stress Kinase Specificity WB. Western blot analysis of HEK293 cell lysates overexpressing mouse MK5 / PRAK or mouse MK2 using goat polyclonal PRAK antibody Cat. # R36080 (0.5 ug/ml). A strong band is detected near 90 kDa in MK5/PRAK-expressing cells, consistent with the expected molecular weight of the PRAK-GFP fusion protein. No detectable cross-reactivity is observed with MK2, supporting selective detection of the p38-regulated stress kinase MAPKAPK5 / PRAK.

PRAK Antibody Stress Signaling IP. Immunoprecipitation analysis using goat polyclonal PRAK antibody Cat. # R36080 (1.5 ug) was performed on lysates from PRAK/ERK3 double knockout mouse embryonic fibroblasts with and without rescued PRAK expression following retroviral transduction. Immunoblot detection with an unrelated PRAK antibody identifies a restored band near 50 kDa in rescued samples, supporting selective immunoprecipitation of MAPKAPK5 / PRAK, a p38-regulated stress-response kinase involved in cellular adaptation pathways.

PRAK Antibody Kidney IHC. Immunohistochemistry analysis of FFPE human kidney tissue stained with goat polyclonal PRAK antibody at 5 ug/ml. Following HIER in pH 6 citrate buffer, distinct AP-red cytoplasmic and apical staining is observed in renal tubular epithelial cells, consistent with expression of MAPKAPK5 / PRAK, a p38-regulated stress kinase involved in cellular adaptation and stress-responsive signaling pathways.