PPM1E Antibody / Protein Phosphatase 1E Antibody (FY13497)

PPM1E Antibody Human Melanoma IHC. Immunohistochemical staining of FFPE human melanoma tissue using PPM1E Antibody / Protein Phosphatase 1E Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer before incubation with PPM1E Antibody at 1 ug/ml. A biotinylated goat anti-rabbit secondary antibody with an SABC detection system and DAB chromogen was used for visualization. PPM1E exhibits predominantly cytoplasmic staining in melanoma cells, consistent with its role as a PP2C family serine/threonine phosphatase that regulates phosphorylation-dependent signaling pathways. This PPM1E Antibody, also called Protein Phosphatase 1E Antibody, is suitable for studies of protein dephosphorylation, MAPK signaling, neuronal signaling, and cancer-associated signal transduction.

PPM1E Antibody Human A431 Cell IF. Immunofluorescent staining of A431 cells using PPM1E Antibody / Protein Phosphatase 1E Antibody (green). Enzymatic antigen retrieval was performed for 15 minutes before incubation with PPM1E Antibody at 2 ug/ml, followed by a DyLight 488-conjugated goat anti-rabbit secondary antibody. Cells were counterstained with DAPI to visualize nuclei. PPM1E displays predominantly cytoplasmic staining with punctate intracellular labeling, consistent with its role as a PP2C family serine/threonine phosphatase that regulates phosphorylation-dependent signaling pathways. This PPM1E Antibody, also called Protein Phosphatase 1E Antibody, is suitable for studies of protein dephosphorylation, MAPK signaling, neuronal signaling, and cancer-associated signal transduction.

PPM1E Antibody Human and Mouse Liver WB. Western blot analysis of human Raji (lane 1), K562 (lane 2), and A431 (lane 3) whole cell lysates, rat liver (lane 4), and mouse liver (lane 5) tissue lysates using PPM1E Antibody / Protein Phosphatase 1E Antibody at 0.5 ug/ml. A specific band is detected at approximately 84 kDa in all samples, consistent with the predicted molecular weight of PPM1E. This PPM1E Antibody, also called Protein Phosphatase 1E Antibody, is suitable for studies of protein dephosphorylation, MAPK signaling, neuronal signaling, and phosphorylation-dependent signal transduction.

PPM1E Antibody Human K562 Cell FACS. Flow cytometry analysis of fixed and permeabilized human K562 cells stained with PPM1E Antibody / Protein Phosphatase 1E Antibody (blue). Cells were blocked with normal goat serum and incubated with PPM1E Antibody at 1 ug/10^6 cells, followed by a DyLight 488-conjugated goat anti-rabbit secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the PPM1E-positive population demonstrates specific intracellular detection. This PPM1E Antibody, also called Protein Phosphatase 1E Antibody, is suitable for flow cytometric analysis of intracellular PPM1E expression.