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Home >> Antibodies >> PPARG Antibody / PPAR gamma

PPARG Antibody / PPAR gamma (F49368)

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  Catalog No Formulation Size Price (USD)  
Image F49368-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49368-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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PPARG antibody flow cytometric analysis of human HepG2 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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PPARG antibody flow cytometric analysis of human HepG2 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
PPARG antibody flow cytometric analysis of human HeLa cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of human 1) fetal liver, 2) lung and 3) placenta lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.
Western blot testing of human lung lysate with PPARG antibody at 1:2000. Expected molecular weight: 54-57 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P37231
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This PPARG antibody is available for research use only.
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Description

PPARG is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity). [UniProt]

Application Notes

Titration of the PPARG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this PPARG antibody.

Storage

Aliquot the PPARG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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