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Home >> Antibodies >> POU5F1 Antibody / Oct4

POU5F1 Antibody / Oct4 (RQ6789)

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  Catalog No Formulation Size Price (USD)  
Image RQ6789 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human HeLa, 2) human placenta, 3) rat heart, 4) rat brain, 5) mouse heart, 6) mouse brain and 7) mouse NIH 3T3 cell lysate with POU5F1 antibody. Predicted molecular weight ~38/30 kDa (isoform A/B), commonly observed at 38-45 kDa.
Western blot testing of 1) human HeLa, 2) human placenta, 3) rat heart, 4) rat brain, 5) mouse heart, 6) mouse brain and 7) mouse NIH 3T3 cell lysate with POU5F1 antibody. Predicted molecular weight ~38/30 kDa (isoform A/B), commonly observed at 38-45 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q01860
Applications Western blot : 1-2ug/ml
Limitations This POU5F1 antibody is available for research use only.
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Description

Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Application Notes

Optimal dilution of the POU5F1 antibody should be determined by the researcher.

Immunogen

Amino acids ENLQEICKAETLVQARK from the human protein were used as the immunogen for the POU5F1 antibody.

Storage

After reconstitution, the POU5F1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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