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Home >> Antibodies >> POU5F1 Antibody

POU5F1 Antibody (F53090)

  Catalog No Formulation Size Price (USD)  
Image F53090-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F53090-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
Western blot analysis of lysate from NCCIT cell line using POU5F1 antibody at 1:1000. Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q01860
Applications Western blot : 1:1000
Limitations This POU5F1 antibody is available for research use only.
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Description

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Application Notes

Titration of the POU5F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This POU5F1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 123-157 amino acids from the Central region of human POU5F1.

Storage

Aliquot the POU5F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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