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Home >> Antibodies >> Pou5f1 Antibody

Pou5f1 Antibody (F52574)

  Catalog No Formulation Size Price (USD)  
Image F52574-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F52574-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 155
Western blot analysis of lysate from zebrafish tissue lysate using Pou5f1 antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q90270
Applications Western blot : 1:1000
Limitations This Pou5f1 antibody is available for research use only.
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Description

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.

Application Notes

Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of zebrafish Pou5f1.

Storage

Aliquot the Pou5f1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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