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Home >> Antibodies >> Positive cofactor 4 Antibody / PC4 / SUB1

Positive cofactor 4 Antibody / PC4 / SUB1 [clone 2D13E3] (RQ7039)

  Catalog No Formulation Size Price (USD)  
Image RQ7039 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human PC-3 cells with Positive cofactor 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Positive cofactor 4 antibody.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) A549 and 4) HepG2 cell lysate with Positive cofactor 4 antibody. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2D13E3
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P53999
Localization Nuclear
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Positive cofactor 4 antibody is available for research use only.
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Description

Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Application Notes

Optimal dilution of the Positive cofactor 4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids N62-L127) was used as the immunogen for the Positive cofactor 4 antibody.

Storage

After reconstitution, the Positive cofactor 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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