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Home >> Antibodies >> PORC-PI-1 Antibody / CDC45

PORC-PI-1 Antibody / CDC45 [clone 6H6] (RQ6278)

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  Catalog No Formulation Size Price (USD)  
Image RQ6278 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human ovarian cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human ovarian cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse thymus with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human 293T cells with PORC-PI-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PORC-PI-1 antibody.
Western blot testing of human 1) K562, 2) HL-60 and 3) HEK293 cell lysate with PORC-PI-1 antibody. Predicted molecular weight ~66 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 6H6
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O75419
Localization Nuclear, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This PORC-PI-1 antibody is available for research use only.
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Description

CDC45 is a protein that in humans is encoded by the CDC45 gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the PORC-PI-1 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids E166-A431) was used as the immunogen for the PORC-PI-1 antibody.

Storage

After reconstitution, the PORC-PI-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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