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Home >> Antibodies >> POMC Antibody / ACTH / Synacthen / N-Terminal

POMC Antibody / ACTH / Synacthen / N-Terminal [clone 57] (V3179)

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  Catalog No Formulation Size Price (USD)  
Image V3179-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3179-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3179SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human pituitary gland with POMC antibody (clone 57). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human pituitary gland with POMC antibody (clone 57). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC: Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH / POMC antibody (clone 57). Required HIER: boil tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Negative control: IHC staining of FFPE human brain tissue with POMC antibody (clone 57). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE Analysis of Purified, BSA-Free POMC Antibody (clone 57). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 57
Purity Protein G affinity chromatography
UniProt P01189
Localization Cytoplasm
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This POMC antibody is available for research use only.
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Description

POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

Application Notes

The optimal dilution of the ACTH / POMC antibody for each application should be determined by the researcher.

1. View the recombinant version of this ACTH antibody.

Immunogen

An N-terminal peptide (within AA 1-24) from the Synacthen/ACTH region of human POMC was used as the immunogen for this POMC antibody.

Storage

Store the POMC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View the recombinant version of this POMC antibody

   2.   View all POMC antibodies

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