Poliovirus receptor related 4 Antibody / NECTIN4 / Extracellular domain [clone NECTIN4/13438R] (V6026)

Immunohistochemistry analysis of Poliovirus receptor related 4 / NECTIN4 antibody (clone NECTIN4/13438R) in human placental tissue. Formalin-fixed, paraffin-embedded placenta demonstrates membranous HRP-DAB brown staining outlining trophoblastic cells and chorionic villi, consistent with cell surface localization of Nectin-4. Background staining is minimal, and hematoxylin counterstain highlights tissue morphology. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Flow cytometry analysis of Poliovirus receptor related 4 / NECTIN4 antibody (clone NECTIN4/13438R) in human T47D breast carcinoma cells. Cells were incubated with recombinant rabbit anti-Nectin-4 monoclonal antibody at dilutions of 1:50 (4 ug/ml; dark red), 1:100 (2 ug/ml; orange), 1:200 (1 ug/ml; light green), and 1:400 (0.5 ug/ml; dark green), followed by goat anti-rabbit IgG-CF488 secondary antibody. CF488 fluorescence intensity (log10 scale, x-axis) is plotted against relative cell count (y-axis). Increasing antibody concentration produces progressive rightward shifts of the fluorescence peak, consistent with dilution-dependent binding to cell-surface Nectin-4. The blue histogram represents the IgG isotype control.

SDS-PAGE Analysis of Purified Poliovirus receptor related 4/NECTIN4 antibody (NECTIN4/13438R). Confirmation of Purity and Integrity of Antibody.