Podocin Antibody / Glomerular Podocyte Marker Antibody (R30383)

Podocin Antibody Human Kidney Glomeruli IHC. Immunohistochemistry staining of FFPE human kidney tissue was performed with Podocin Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. HRP-conjugated secondary antibody and DAB were used for detection. Strong podocin staining is observed in multiple renal glomeruli, prominently highlighting the glomerular capillary network and podocyte-associated structures. This Podocin Antibody demonstrates the characteristic glomerular localization of NPHS2 and supports its use as a glomerular podocyte marker.

Podocin Antibody Human Kidney Glomerulus IHC. Immunohistochemistry staining of FFPE human kidney tissue was performed with Podocin Antibody at 2 ug/ml following heat-induced epitope retrieval in pH 8 EDTA buffer. HRP-conjugated secondary antibody and DAB were used for detection. Strong podocin staining is observed within the renal glomerulus, highlighting the glomerular capillary network and podocyte-associated structures. This Podocin Antibody demonstrates localization of NPHS2 in human kidney and supports its use as a glomerular podocyte marker.

Podocin Antibody Rat Kidney Glomeruli IHC. Immunohistochemistry staining of FFPE rat kidney tissue was performed with Podocin Antibody at 1 ug/ml following heat-induced epitope retrieval in pH 6 citrate buffer for 20 min. Biotinylated goat anti-rabbit IgG and streptavidin-biotin complex with DAB were used for detection. Strong podocin staining is selectively localized to renal glomeruli, with minimal staining in the surrounding tubular tissue. This Podocin Antibody demonstrates the characteristic glomerular distribution of NPHS2 and supports its use as a glomerular podocyte marker.

Podocin Antibody Mouse Kidney Glomeruli IHC. Immunohistochemistry staining of FFPE mouse kidney tissue was performed with Podocin Antibody at 1 ug/ml following heat-induced epitope retrieval in pH 6 citrate buffer for 20 min. Biotinylated goat anti-rabbit IgG and streptavidin-biotin complex with DAB were used for detection. Strong podocin staining is selectively localized to renal glomeruli, with little staining observed in the surrounding tubular tissue. This Podocin Antibody demonstrates the characteristic glomerular distribution of NPHS2 and supports its use as a glomerular podocyte marker.

Podocin Antibody Rat Kidney Glomerulus IHC. Immunohistochemistry staining of frozen rat kidney tissue was performed with Podocin Antibody at 1 ug/ml overnight at 4oC. Biotinylated goat anti-rabbit IgG and streptavidin-biotin complex with DAB were used for detection. Strong podocin staining is localized to the renal glomeruli, with minimal staining in the surrounding tubular tissue. This Podocin Antibody demonstrates the characteristic glomerular distribution of NPHS2 and supports its use as a glomerular podocyte marker.

Podocin Antibody Rat Kidney WB. Western blot analysis was performed with Podocin Antibody on rat kidney tissue lysate under reducing conditions. The antibody was used at 0.5 ug/ml overnight at 4oC. A specific band is detected at approximately 45 kDa, consistent with the expected molecular weight of approximately 42 kDa for podocin/NPHS2. This Podocin Antibody demonstrates detection of NPHS2 in rat kidney tissue and supports its use for studies of glomerular podocyte biology and slit diaphragm organization.

Podocin Antibody Human 293T Cell WB. Western blot analysis was performed with Podocin Antibody on human 293T whole cell lysate under reducing conditions. The antibody was used at 0.5 ug/ml overnight at 4oC. A prominent band is detected at approximately 45 kDa, close to the expected molecular weight of approximately 42 kDa for podocin/NPHS2. Additional bands are observed at approximately 65-70 kDa and 30 kDa. This Podocin Antibody demonstrates detection of NPHS2 in human 293T cell lysate.