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Home >> Antibodies >> Platelet Endothelial Cell Adhesion Molecule 1 Antibody / PECAM-1

Platelet Endothelial Cell Adhesion Molecule 1 Antibody / PECAM-1 [clone MSVA-031M] (V5967)

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  Catalog No Formulation Size Price (USD)  
Image V5967-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
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V5967-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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Immunohistochemistry analysis of Platelet Endothelial Cell Adhesion Molecule 1 / PECAM-1 antibody in human tissue microarrays. IHC was performed on FFPE human normal and cancer tissue microarrays using Platelet Endothelial Cell Adhesion Molecule 1 / PECAM-1 antibody (clone MSVA-031M). HRP-DAB brown chromogenic signal is predominantly localized to vascular endothelial cells across multiple normal tissues, highlighting membranous staining along blood vessel linings. In cancer specimens, staining delineates tumor-associated vasculature with variable microvessel density depending on tumor type. Non-endothelial stromal and epithelial components generally show minimal background staining. Nuclei are counterstained blue. The observed staining distribution is consistent with established endothelial expression patterns reported in public expression datasets, including the Human Protein Atlas
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name MSVA-031M
UniProt P16284
Localization Cell junction, Cell membrane, Membrane raft
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This Platelet Endothelial Cell Adhesion Molecule 1/PECAM-1 antibody is available for research use only.
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Description

Platelet Endothelial Cell Adhesion Molecule 1 antibody, also known as PECAM-1 antibody and CD31 antibody, recognizes a transmembrane adhesion receptor of the immunoglobulin superfamily encoded by the PECAM1 gene on chromosome 17q23.3. Platelet Endothelial Cell Adhesion Molecule 1 is predominantly localized to the plasma membrane of vascular endothelial cells, where it is highly enriched at intercellular junctions. It is also expressed on platelets, monocytes, neutrophils, and subsets of lymphocytes. Within vascular tissues, PECAM-1 serves as a defining endothelial marker due to its consistent membranous distribution along vessel walls.

Functionally, Platelet Endothelial Cell Adhesion Molecule 1 mediates homophilic adhesion between adjacent endothelial cells and plays a pivotal role in leukocyte transmigration during inflammatory responses. Through regulated junctional interactions, it allows immune cells to pass between endothelial cells while preserving vascular integrity. PECAM-1 is also involved in platelet-endothelial interactions and contributes to thromboregulatory processes. Platelet Endothelial Cell Adhesion Molecule 1 antibody is widely used to visualize blood vessels, assess angiogenesis, and evaluate microvessel density in tumor research.

Structurally, PECAM-1 contains six extracellular immunoglobulin-like domains, a single-pass transmembrane segment, and a cytoplasmic tail with signaling motifs capable of recruiting phosphatases and adaptor proteins. These signaling interactions influence cytoskeletal organization, mechanotransduction, and integrin-associated pathways that respond to shear stress within the vasculature. Through these mechanisms, PECAM-1 integrates adhesive and signaling functions essential for vascular homeostasis.

Dysregulation of PECAM1 expression or altered localization has been associated with vascular inflammation, atherosclerosis, ischemic injury, and tumor-associated neovascularization. Increased endothelial staining often correlates with enhanced angiogenic activity in malignant tissues. Recombinant monoclonal clone MSVA-031M recognizes Platelet Endothelial Cell Adhesion Molecule 1 and is suitable for detecting PECAM-1 expression in relevant research applications.

Application Notes

1. Optimal dilution of the Platelet Endothelial Cell Adhesion Molecule 1/PECAM-1 antibody should be determined by the researcher.

2. This Platelet Endothelial Cell Adhesion Molecule 1/PECAM-1 antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 625-738) of human CD31 protein (exact sequence is proprietary) was used as the immunogen for the Platelet Endothelial Cell Adhesion Molecule 1/PECAM-1 antibody.

Storage

Platelet Endothelial Cell Adhesion Molecule 1/PECAM-1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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