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Home >> Antibodies >> PLAP Antibody / Placental alkaline phosphatase

PLAP Antibody / Placental alkaline phosphatase (R32610)

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  Catalog No Formulation Size Price (USD)  
Image R32610 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with PLAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with PLAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placental tissue with PLAP antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot testing of human placenta lysate with PLAP antibody. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.
Flow cytometry testing of human SiHa cells with PLAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLAP antibody.
Immunofluorescent staining of FFPE human placental tissue with PLAP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P05187
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This PLAP antibody is available for research use only.
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Description

Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Application Notes

Optimal dilution of the PLAP antibody should be determined by the researcher.

Immunogen

Amino acids 403-433 (KARDRKAYTVLLYGNGPGYVLKDGARPDVTE-human) were used as the immunogen for the PLAP antibody.

Storage

After reconstitution, the PLAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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