PLAP Antibody / Germ Cell Tumor Marker Antibody (R32610)

PLAP Antibody HeLa IF. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human HeLa cells stained with PLAP Antibody demonstrates cytoplasmic and membranous fluorescence signal (green) consistent with expression of placental alkaline phosphatase / ALPP in epithelial-derived tumor cells. This germ cell tumor marker antibody highlights peripheral membranous and cytoplasmic localization patterns associated with placental alkaline phosphatase expression in transformed epithelial cell populations. Nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 min prior to antibody incubation.

PLAP Antibody Placenta IHC. Immunohistochemistry analysis of FFPE human placenta tissue stained with PLAP Antibody demonstrates strong circumferential membranous HRP-DAB brown staining outlining trophoblast-lined chorionic villi. The staining pattern is consistent with expression of placental alkaline phosphatase / ALPP in syncytiotrophoblast-associated epithelial compartments and highlights the characteristic membrane localization of this germ cell tumor-associated differentiation marker. HIER: steam section in pH8 EDTA buffer for 20 min.

PLAP Antibody Placenta WB. Western blot analysis of human placenta tissue lysate using PLAP Antibody detects a strong band at approximately 65-70 kDa, consistent with placental alkaline phosphatase / ALPP. Although the predicted molecular weight of PLAP is approximately 58 kDa, the protein is routinely visualized at higher apparent molecular weights due to extensive glycosylation and post-translational processing characteristic of this glycosylphosphatidylinositol-anchored alkaline phosphatase family member and germ cell tumor-associated differentiation marker.

PLAP Antibody HeLa FACS. Flow cytometry analysis of human HeLa cells stained with PLAP Antibody demonstrates a distinct right-shifted fluorescence population relative to the isotype control, consistent with cell-associated expression of placental alkaline phosphatase / ALPP in epithelial-derived tumor cells. This germ cell tumor marker antibody supports detection of membrane-associated PLAP expression in transformed cellular populations. Red=cells alone, Green=isotype control, Blue=PLAP antibody.

PLAP Antibody Placenta IF. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human placenta tissue stained with PLAP Antibody demonstrates strong membranous fluorescence signal outlining chorionic villi and syncytiotrophoblast-associated epithelial surfaces (red), consistent with expression and surface localization of placental alkaline phosphatase / ALPP in trophoblastic cellular compartments. This germ cell tumor marker antibody highlights the characteristic membrane distribution of PLAP within placental tissue. Nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 min prior to antibody incubation.