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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PLAP Antibody Rabbit Polyclonal / Placental alkaline phosphatase (R32610)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R32610 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | P05187 |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells |
| Limitations | This PLAP antibody is available for research use only. |
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Placental alkaline phosphatase is a glycosylphosphatidylinositol-anchored membrane enzyme encoded by the ALPP gene and commonly referred to as PLAP. The PLAP Antibody Rabbit Polyclonal is developed to detect this trophoblast-associated alkaline phosphatase in research applications focused on placental biology and germ cell tumor expression. ALPP is located on chromosome 2q37 and belongs to the alkaline phosphatase family of homodimeric metalloenzymes that catalyze the hydrolysis of phosphate monoesters under alkaline conditions.
In normal physiology, PLAP is strongly expressed on the apical membrane of syncytiotrophoblasts within placental chorionic villi. Because the enzyme is anchored to the outer surface of the plasma membrane through a glycosylphosphatidylinositol linkage, immunohistochemical detection typically demonstrates crisp membranous staining in trophoblastic cells. Most non-placental adult tissues show minimal expression, supporting its role as a lineage-associated marker in tissue-based studies.
In oncologic research, PLAP expression has been extensively evaluated in germ cell tumors, particularly seminoma and embryonal carcinoma, as well as in certain trophoblastic neoplasms. Strong membranous and occasional cytoplasmic staining patterns are commonly observed in tumor epithelial cells in these contexts, whereas most non-germ cell carcinomas display little to no staining. This relatively restricted expression profile enhances its value in research panels assessing germ cell differentiation and trophoblastic lineage markers.
Although placental alkaline phosphatase shares structural similarity with tissue-nonspecific and intestinal alkaline phosphatase isoenzymes, its trophoblastic and germ cell-associated distribution is more defined. A rabbit polyclonal PLAP antibody supports detection of ALPP in formalin-fixed, paraffin-embedded specimens and other protein expression studies investigating membrane-associated enzyme localization.
Optimal dilution of the PLAP antibody should be determined by the researcher.
Amino acids 403-433 (KARDRKAYTVLLYGNGPGYVLKDGARPDVTE-human) were used as the immunogen for the PLAP antibody rabbit polyclonal.
After reconstitution, the PLAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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