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Home >> Antibodies >> Plakophilin 1 Antibody

Plakophilin 1 Antibody [clone 10B2] (V3392)

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  Catalog No Formulation Size Price (USD)  
Image V3392-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3392-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3392SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.
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Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Plakophilin 1 Antibody (clone 10B2). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 10B2
Purity Protein G affinity chromatography
UniProt Q13835
Localization Cell surface, nuclear
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Limitations This Plakophilin 1 antibody is available for research use only.
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Description

Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1). Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Application Notes

Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.

Immunogen

A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.

Storage

Store the Plakophilin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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