Junction Plakoglobin Antibody / Plakoglobin Polyclonal Antibody (R31599)

Junction Plakoglobin Antibody Western Blot of Human, Mouse and Rat Samples. Western blot testing of rat brain (lane 1), rat heart (lane 2), rat thymus (lane 3), rat RH35 cells (lane 4), human HeLa cells (lane 5), human COLO-320 cells (lane 6), human HepG2 cells (lane 7), mouse HEPA1-6 cells (lane 8) and human MCF7 cells (lane 9) with Junction Plakoglobin Antibody. A strong, discrete band is detected at approximately 86 kDa across all nine samples, matching the expected molecular weight of plakoglobin. The consistent target band and minimal additional reactivity support cross-species detection with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody Western Blot of Recombinant Human Protein. Western blot testing of recombinant human plakoglobin protein (0.5 ng) with Junction Plakoglobin Antibody. A single prominent immunoreactive band is visible in the tested sample, demonstrating direct recognition of recombinant target protein. This recombinant protein western blot provides an additional assessment of target recognition by the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Human Intestine Cancer IHC. Immunohistochemistry staining of FFPE human intestine cancer tissue with Junction Plakoglobin Antibody. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min. Brown staining is prominent along the borders of tumor cells, producing a distinct membranous and junction-associated pattern with comparatively limited nuclear signal. The localization is consistent with plakoglobin at epithelial cell-cell contacts and supports tissue detection with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Mouse Intestine IHC. Immunohistochemistry staining of FFPE mouse intestine tissue with Junction Plakoglobin Antibody. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min. Strong brown staining is concentrated along the lateral and apical borders of intestinal epithelial cells, clearly delineating glandular structures while surrounding stromal areas show substantially less signal. This junction-associated epithelial distribution demonstrates mouse tissue detection with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Rat Intestine IHC. Immunohistochemistry staining of FFPE rat intestine tissue with Junction Plakoglobin Antibody. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min. Strong brown staining prominently outlines the intestinal epithelium, with particularly clear labeling along epithelial cell borders and luminal surfaces. The distinct junction-associated pattern demonstrates endogenous plakoglobin localization in rat intestine using the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Frozen Rat Intestine IHC. Immunohistochemistry staining of frozen rat intestine tissue with Junction Plakoglobin Antibody. Brown signal is concentrated within the intestinal epithelium and prominently delineates epithelial cell borders throughout the glandular structures. The staining pattern is consistent with the localization of plakoglobin at intercellular junctions and demonstrates detection in frozen tissue using the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Human A431 Epidermoid Carcinoma Cells IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells with Junction Plakoglobin Antibody (green) and DAPI nuclear stain (blue). HIER was performed by steaming in pH 6 citrate buffer for 20 min. Green fluorescence is concentrated at cell borders and regions of contact between adjacent cells, while nuclei remain blue. The junction-associated distribution supports cellular localization of plakoglobin with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Human MCF7 Breast Cancer Cells ICC. Immunocytochemistry staining of FFPE human MCF7 breast cancer cells with Junction Plakoglobin Antibody. HIER was performed by boiling in pH 6 10mM citrate buffer for 20 min followed by cooling before testing. Brown staining is concentrated predominantly along cell borders and at contacts between neighboring cells, producing a junction-associated pattern consistent with plakoglobin localization. This cellular staining supports detection with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody in Human A431 Epidermoid Carcinoma Cells FACS. Flow cytometry testing of human A431 epidermoid carcinoma cells with Junction Plakoglobin Antibody at 1 ug/million cells following blocking with goat sera. Cells alone are shown in red, the isotype control in green, and antibody staining in blue. The blue population exhibits a pronounced rightward fluorescence shift relative to both controls, demonstrating detection of endogenous plakoglobin with the Plakoglobin Polyclonal Antibody.

Junction Plakoglobin Antibody Western Blot of Human Cell Lines and Rat Heart. Western blot testing of human 293T cells (lane 1), MCF7 cells (lane 2), T-47D cells (lane 3) and rat heart tissue (lane 4) with Junction Plakoglobin Antibody. A predominant band is detected near the expected molecular weight of approximately 86 kDa in all four samples, with particularly strong signal in T-47D cells and rat heart. Minor lower-molecular-weight reactivity is also visible in some lanes. The predominant target-sized signal further supports detection with the Plakoglobin Polyclonal Antibody.