Plakoglobin Antibody 15F11 / Gamma Catenin Monoclonal Antibody [clone 15F11] (V3205)

Plakoglobin Antibody 15F11 in Human HeLa Cells IF. Immunofluorescence staining of human HeLa cells with Plakoglobin Antibody clone 15F11 (green) and Reddot nuclear stain (red). The green signal strongly outlines cell borders, with particularly prominent labeling at contacts between adjacent cells. The merged image demonstrates the expected junction-associated distribution of plakoglobin with comparatively little nuclear signal. This distinct cell-cell border pattern supports detection of junctional JUP with the Gamma Catenin Monoclonal Antibody.

Plakoglobin Antibody 15F11 Western Blot of Six Human Cell Lines. Western blot testing of human HT20 (lane 1), HEK293 (lane 2), A431 (lane 3), MCF7 (lane 4), HEPG2 (lane 5) and A549 (lane 6) cell lysates with Plakoglobin Antibody clone 15F11. A strong, discrete band is detected at approximately 80-85 kDa in all six samples, consistent with the predicted molecular weight of 80-87 kDa for plakoglobin. Signal intensity is relatively consistent across the panel, with minimal additional banding visible. This broad endogenous detection supports the performance of the Gamma Catenin Monoclonal Antibody for western blot analysis.

Plakoglobin Antibody 15F11 in Human MCF7 Breast Cancer Cells FACS. Flow cytometry testing of PFA-fixed human MCF7 breast cancer cells with Plakoglobin Antibody clone 15F11. The antibody-stained population (blue) shows a clear rightward fluorescence shift relative to the isotype control (red), demonstrating detection of endogenous plakoglobin in fixed cells. The separation of the two distributions supports cellular detection of JUP with the Gamma Catenin Monoclonal Antibody.

Plakoglobin Antibody 15F11 Western Blot of HeLa and SK-BR-3 Cells. Western blot testing of human HeLa (lane 1) and SK-BR-3 (lane 2) cell lysates with Plakoglobin Antibody clone 15F11. Both samples show a predominant band at approximately 80-90 kDa, consistent with the predicted molecular weight of 80-87 kDa for plakoglobin. The HeLa and SK-BR-3 samples show slightly different apparent migration within this range, while additional banding is minimal. This two-cell-line analysis further supports target detection with the Gamma Catenin Monoclonal Antibody.

Plakoglobin Antibody 15F11 Shows Junctional Staining in Human Cervix IHC. Immunohistochemistry staining of FFPE human cervix tissue with Plakoglobin Antibody clone 15F11. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. Strong brown staining prominently outlines the borders of epithelial cells, producing a characteristic intercellular junction pattern, while nuclei remain largely unstained. The localization is consistent with plakoglobin at epithelial cell-cell junctions and demonstrates tissue detection with the Gamma Catenin Monoclonal Antibody.