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Home >> Antibodies >> Plagl1 Antibody / Lot1 / Mac

Plagl1 Antibody / Lot1 / Mac (RQ7517)

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  Catalog No Formulation Size Price (USD)  
Image RQ7517 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE mouse RM1 cells with Plagl1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE mouse RM1 cells with Plagl1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of mouse heart tissue lysate with Plagl1 antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of mouse ANA-1 cells with Plagl1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Plagl1 antibody.
Availability 1-3 business days
Species Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt B7ZP38
Localization Nuclear, possibly cytoplasmic (ER)
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Plagl1 antibody is available for research use only.
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Description

Zinc finger protein PLAGL1 (Pleiomorphic adenoma-like protein 1), also called Tumor suppressor ZAC and LOT 1 (Lost on transformation 1) is a protein that in humans is encoded by the PLAGL1 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within regulation of gene expression and skeletal muscle cell differentiation. Predicted to be located in Golgi apparatus and nuclear body. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; heart; and sensory organ. Orthologous to human PLAGL1 (PLAG1 like zinc finger 1).

Application Notes

Optimal dilution of the Plagl1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant mouse protein (amino acids M1-Q527) was used as the immunogen for the Plagl1 antibody.

Storage

After reconstitution, the Plagl1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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