PRKCA Antibody / PKC alpha Rabbit Polyclonal Antibody (F54980)

PRKCA Antibody by IHC in human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human lung carcinoma tissue using PKC alpha Rabbit Polyclonal Antibody demonstrates brown immunoreactivity in tumor cells, with variable staining intensity across the tissue section. Positive staining is primarily cytoplasmic, while hematoxylin counterstaining highlights cell nuclei. Heat-induced epitope retrieval was performed by steaming the section in citrate buffer, pH 6.0, for 20 minutes, followed by cooling before antibody staining. The observed immunoreactivity illustrates PKC alpha expression in human lung carcinoma tissue.

PRKCA Antibody by IF in human HeLa cells. Immunofluorescence staining of HeLa cells using PKC alpha Rabbit Polyclonal Antibody demonstrates green fluorescence concentrated along the cytoplasmic and perinuclear regions, with additional signal surrounding the nuclei. Actin is visualized in red, outlining the cellular architecture, while DAPI counterstaining identifies nuclei in blue. The merged image shows PKC alpha immunoreactivity distributed predominantly outside the nuclei, consistent with cytoplasmic localization of this signaling kinase.

PRKCA Antibody by WB in mouse NIH 3T3 cells. Western blot analysis of mouse NIH 3T3 cell lysate using PKC alpha Rabbit Polyclonal Antibody demonstrates a distinct immunoreactive band migrating near 77 kDa, consistent with the predicted molecular weight of protein kinase C alpha. The blot contains a single sample lane with a prominent band positioned between the 72 and 95 kDa molecular weight markers. The observed migration is consistent with detection of full-length PRKCA, a conventional protein kinase C family member involved in calcium- and diacylglycerol-dependent signaling. This result demonstrates recognition of the endogenous target in a mouse fibroblast-derived cell line.

PRKCA Antibody by FACS in human CEM cells. Flow cytometry analysis of human CEM cells using PKC alpha Rabbit Polyclonal Antibody demonstrates a fluorescence distribution shifted toward higher intensity relative to the isotype control. The antibody-stained population is shown in green, while the isotype control is shown in blue. The separation between the distributions indicates detectable PKC alpha immunoreactivity in the tested cell population. This experiment illustrates antibody recognition of PRKCA in human CEM cells under the reported flow cytometry conditions.